Figure 3

Morphological changes of pulmonary tissue in HDM-sensitized B6 mouse. (a) Optical microscope image of Bronchi (Br) and accompanying blood artery (Ar), HE stained. (b) SEM image of Br and Ar, Grocott stained. EM, 10kv, 2.5 nm. (c) Magnified SEM image of Br and Ar. (d) Optical microscope image of another Br and Ar, HE stained. (e) SEM image of Br and Ar, Grocott stained. f) Magnified image of Br and Ar. (a–c) HDM-sensitized B6 mouse developed fibrosis surrounding the entire bronchus, especially near the artery, and mucus production only from the same area. (d–f) Mucus vesicles are being produced from bronchial epithelial cells. All scale bars show 200 μm length.