Figure 4 | Scientific Reports

Figure 4

From: Effects of acute administration of trimethylamine N-oxide on endothelial function: a translational study

Figure 4

TMAO injection does not alter rat aortic eNOS-NO molecular signalling. In the aorta of rats treated with TMAO before sacrifice, key vasodilatory signalling and stress-induced signalling were unchanged. (A) PKA phosphorylation, (B) Akt phosphorylation, (C) PKCbII phosphorylation, (D) SAPK-JNK1 and (E). SAPK-JNK2 phosphorylations are not affected. (F) eNOS threonine 495 inhibitory phosphorylation and (G) eNOS serine 1177 activatory phosphorylation. (H) Diagram of the addressed eNOS-NO signalling. Rat data: Original blots/gels are presented in Supplementary Fig. 4. Unpaired T-test, n = 6 in each group. TMAO: trimethylamine N-oxide, GLP-1: glucagon-like peptide-1, eNOS: endothelial nitric oxide synthase, PKA: protein kinase A, Akt: protein kinase B, PKCßII: protein kinase C ßII, SAPK-JNK: stress activated protein kinases/Jun-amino terminal kinases.

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