Figure 1 | Scientific Reports

Figure 1

From: Remuscularization with triiodothyronine and β1-blocker therapy reverses post-ischemic left ventricular dysfunction and adverse remodeling

Figure 1

T3-induced ERK1/2 phosphorylation and cyclin expression in adult ventricular CMs is potentiated by β1-AR siRNA. (A) Schematic showing the protocol for β1-AR siRNA and T3 administration and analysis times. (B) Representative immunoblots show expression of indicated proteins in lysates obtained from CMs at 40 h after T3 administration. (C–M) Quantitative data obtained from analysis of immunoblots for β1-AR (C), cyclin D1 (D), cyclin A2 (E), cyclin B1 (F), ECT2 (G), p-MEK1/2 (H), MEK1/2 (I), p-ERK1 (J), ERK1 (K), p-ERK2 (L) and ERK2 (M). The data were normalized with loading control (GAPDH) and data were expressed as relative value for scrambled control siRNA without T3 therapy. Immunoblots are representative of 4 biologically independent replicates. Data are mean ± SEM. ***P < 0.001.

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