Figure 4

Structural comparison of the GTD^TcdA–RhoA and the GTD^{TcdB-VPI10463}–Cdc42 complexes. (A) K448 of GTD^TcdA (hot pink) but not the equivalent E449 of GTD^TcdB (pale yellow, PDB code: 7S0Y) form a salt bridge with E40 (cyan) of RhoA and potentially D38 of Cdc42 (pale yellow). (B) Residues A377 and L378 on the lower clamp of GTD^TcdA (hot pink) interact with the switch I of RhoA better than the equivalent N378 and S379 on GTD^{TcdB-VPI10463} (pale yellow, PDB code: 7S0Y). (C) Examining the interactions between the pre-switch I of RhoA (cyan) and the lower clamp of GTD^TcdA (hot pink) in the GTD^TcdA–RhoA complex when compared to the GTD^{TcdB-VPI10463}–Cdc42 complex (pale yellow) and RhoA·GDP (slate, PDB code: 1FTN). (D) Comparing the interactions in the switch II area for the GTD^TcdA–RhoA (hot pink and cyan, respectively) and the GTD^{TcdB-VPI10463}–Cdc42 (pale yellow) complexes.