Figure 2 | Scientific Reports

Figure 2

From: The Minichromosome Maintenance Complex Component 2 (MjMCM2) of Meloidogyne javanica is a potential effector regulating the cell cycle in nematode-induced galls

Figure 2

MjMCM2 expression pattern throughout M. javanica's life cycle. MjMCM2 transcripts from five different nematode developmental stages (eggs, preparasitic (pp) J2s, parasitic (p) J2s, J3–4s, and mature females) were subjected to qRT-PCR analysis. The transcripts were normalized against two endogenous nematode reference genes, 18S and EF-1α. Each reaction was performed in triplicate and results represent the mean of three replicates of one independent biological replicate. Data represent the mean relative expression and standard error obtained from one independent biological experiment. Different letters above the bars denote a significant difference (P ≤ 0.05, ANOVA) between samples by Tukey–Kramer multiple comparison test. The experiment was repeated twice for each housekeeping gene and similar results were obtained.

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