Figure 3 | Scientific Reports

Figure 3

From: The Minichromosome Maintenance Complex Component 2 (MjMCM2) of Meloidogyne javanica is a potential effector regulating the cell cycle in nematode-induced galls

Figure 3

Localization of MjMCM2 mRNA using a gene-specific MjMCM2-Cy5 cDNA-labeled probe (red) visualized in dissected M. javanica juvenile nematodes exposed to the oxylipin 9-HOT. M. javanica J2s were counterstained with DAPI (nuclei; blue). (a) J2s exposed to oxylipin 9-HOT and then subjected to FISH. MjMCM2 mRNA fluorescence was visualized by fluorescence microscopy. Top: DAPI staining (left) and hybridization signal in red indicated by arrows (right). Bottom: M. javanica visualized by differential interference contrast (DIC) microscopy (left) and overlaid images with DAPI staining and hybridization signal (right). (b) Control J2s were subjected to FISH in 0.01 M MES buffer without exposure to 9-HOT, resulting in no hybridization signal. Samples were visualized under an IX81Olympus FluoView500 confocal microscope. Scale bar = 20 µm.

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