Figure 4 | Scientific Reports

Figure 4

From: The Minichromosome Maintenance Complex Component 2 (MjMCM2) of Meloidogyne javanica is a potential effector regulating the cell cycle in nematode-induced galls

Figure 4

Subcellular localization of MjMCM2 tagged with eGFP in N. benthamiana leaves and apparent co-localization with endoplasmic reticulum, Golgi and plasma membrane. Organelle markers: mCherry–endoplasmic reticulum (ER-Rb; 35S::mCherry-HDEL), mCherry–Golgi (GmMan1-RFP) and mCherry–cytoplasmic membrane (aquaporin PIP2A:RFP). Samples were analyzed with HyD (hybrid) detectors ranging from 500 to 530 and 580 to 650 nm, respectively. Leaves were infiltrated with a 1:1 mixture of A. tumefaciens GV3010 containing (a–c) MjMCM2–eGFP (green) or ER-Rb (red); (d–f) MjMCM2–eGFP or Golgi-Rb(GmMan1-RFP) (red); (g–i) MjMCM2–eGFP or membrane-Rb(aquaporin PIP2A:RFP) (red); (j–l) MjMCM2–eGFP or membrane-Rb(aquaporin PIP2A:RFP) (red); (a,d,g,j) Images acquired with BA505–525 filter for GFP. (b,e,h,k) Images acquired with filter BA560IF for RFP. (c,f,i,l) Sequential acquisition and fusion of images acquired with both filters, overlap of GFP and RFP signals displayed in yellow. Scale bars: (c) 10 µm; (f) 5 µm; (i) 20 µm; (l) 10 µm. (b) Subcellular location results of MjMCM2 as predicted using WoLF PSORT (https://www.genscript.com/wolf-psort.html; and LOCALIZER (http://localizer.csiro.au/).

Back to article page