Figure 1 | Scientific Reports

Figure 1

From: LGR5 is a conserved marker of hair follicle stem cells in multiple species and is present early and throughout follicle morphogenesis

Figure 1

LGR5 is expressed in the hair follicle outer root sheath in the lower bulge in humans, pigs and mice. (A) Schematic of H2B-GFP knock-in at the porcine LGR5 locus. 500 base pair regions of homology on either side of the CRISPR target site at the start codon were included in the homology directed repair template. (B) PCR amplification of DNA from a colony containing the knock-in, from left: 200 bp DNA Ladder, 5′ junction (allele #1), 3′ junction (allele #1), unedited genomic allele (allele #2) (full gel in Fig. S1). (C) Sequencing of LGR5 allele #1 and allele #2 shows the endogenous locus of allele #1 remains unaltered and expresses the LGR5 protein, while allele #2 encodes H2B-GFP instead. (D) LGR5 transcript expression detected by fluorescent in situ hybridization to a pig LGR5 probe (LGR5-ISH) correlates with LGR5-H2BGFP expression in the skin of LGR5-H2B-GFP pigs. (E–J) Hair follicle structure and LGR5 mRNA localization (RNAscope) in follicles from humans (E, H), pigs (F, I) and mice (G, J). Scale bar represents 200 μM.

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