Figure 2 | Scientific Reports

Figure 2

From: LGR5 is a conserved marker of hair follicle stem cells in multiple species and is present early and throughout follicle morphogenesis

Figure 2

LGR5-H2BGFP cells give rise to transit amplifying populations in the hair matrix in anagen. (A–C) 20 × magnification confocal image of base of anagen hair follicle depicting LGR5-H2BGFP expression as compared with DAPI. Intensity profile of each fluorescence along the white arrow is plotted in (D), which is summarized by the fit line. (E) Quantification of slope from profile plots of GFP or DAPI profiles, with profile line drawn from base toward inner root sheath, showing that LGR5-H2BGFP intensity is diluted significantly while DAPI remains relatively unchanged, n = 12 follicles from 3 different pigs. IHC Staining to detect (F) KI67 (G) Keratin 40 (KRT40) or (H) LGR5-H2BGFP signal dilution as LGR5 cells give rise to the proliferating transit amplifying cells (KI67). This population then differentiating into the inner root sheath or hair shaft (KRT40). (I) Merge. Scale bar represents 100 μM.

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