Figure 3 | Scientific Reports

Figure 3

From: LGR5 is a conserved marker of hair follicle stem cells in multiple species and is present early and throughout follicle morphogenesis

Figure 3

LGR5-H2BGFP epidermal cells express known stem cell and epidermal markers. Confocal microscopy images of hair follicles following antibody staining with anti-GFP and known skin markers and overlaid with DAPI. (A) Keratin 10 (KRT10), (B) Keratin 14 (KRT14), (C) Keratin 27 (KRT27), (D) CD200, (E) Aquaporin 3 (AQP3), (F) Aquaporin 5 (AQP5). Scale bar represents 200 μM. (G) Relative expression of known stem cell markers based on delta-delta Ct analysis of RT-qPCR from LGR5-H2BGFP-high (LGR5-HI, light gray), LGR5-H2BGFP-low (LGR5-LO, dark gray), or LGR5-H2BGFP negative (LGR5-NEG, black) populations after flow sorting. Each sample is normalized to ACTB and GAPDH and then to unsorted epidermis, n = 3 pigs. ANOVA followed by Tukey’s honestly significant difference (HDS) test ***P < 0.005, *P < 0.05, error bars indicate standard deviation.

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