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Figure 1

From: Reconstruction of functional human epidermis equivalent containing 5%IPS-derived keratinocytes treated with mitochondrial stimulating plant extracts

Figure 1

Mitochondrial contribution to the energetic metabolism of fibroblasts and their differentiated counterparts, iPSCs and KiPS. (A) The comparison of basal respiration results from Seahorse XF96 analyzer. Fibroblasts (NHF) from a 20-year old (white bars) and a 40-year old donor (black bars) were derived into iPSCs (IPS) and then these cells were differentiated into KiPS (KIPS) (see Materials and Methods). KIPS were analysed at day 8 after their differentiation (n = 2 experiments and 5 replicates). Mitochondrial inhibitors were sequentially injected; and basal respiration, which is the difference between the basal OCR and the oligomycin repressed OCR was determined. (B) Spare respiratory capacity is the calculated difference between the FCCP-induced OCR and the basal OCR. Statistical significance was determined by a Student’s t test. **P < 0.01 and *P < 0.05, errors bars: SEM.

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