Figure 8

Subcellular localization of SlNIP-1 protein and its mutations of site-specific deletion. The roots of seven-day-old seedlings were used and the fluorescence were monitored. Bars 100 µm. Vectors of 35::SlNIP-1-GFP, 35::SlNIP-1Δ140V-GFP, 35::SlNIP-1Δ141T-GFP, 35::SlNIP-1Δ142K-GFP, 35::SlNIP-1Δ143N-GFP, and 35::SlNIP-1Δ144V-GFP were introduced into Arabidopsis (Arabidopsis thaliana) ecotype Columbia mediated by Agrobacterium tumefaciens strain GV3101. T1 transgenic plants were selected by genotyping.