Figure 2
CPC population maintenance reduced by RTKIs. (a) CPC cell cycle activity was reduced by RTKIs: 10 µM IM, 1.25 µM SM or 10 µM ST for 24 h reduced cells in S, G2 and M phases (n = 3). (b) Representative Ki67 ICC images (scale bar = 50 µm). (c) Ki67 protein expression reduced relative to control CPCs by 10 µM IM, 1.25 µM SM or 10 µM ST (n = 3). (d) Representative BrdU ICC images (scale bar = 50 µm). (e) CPC proliferation reduced by RTKIs: percentage BrdUpos CPCs reduced by 10 µM IM, 1.25 µM SM or 10 µM ST over 48 h (n = 3). (f) Real-time qPCR analysis of CPC ‘stemness’ and CPC marker gene expression: reduction in c-kit expression by 5 µM ST after 7 days (n = 3). (g) Downregulation of c-kit protein expression by 5 µM ST after 7 days. All data are mean ± SEM; significance was determined by ANOVA with Holm-Šídák post hoc test to identify differences (**p < 0.01; ***p < 0.001). Blots cropped to relevant data, with optical density normalized to the loading control protein; full blot images presented in Supplementary Fig. 6.
