Table 1 Spectral characteristics and performance of the selected BFPs.

Ex max (nm)

402²⁵

383²¹

399²⁰

402

403

Em max (nm)

467²⁵

448²¹

454²⁰

456

456

Relative brightness in HEK cells^a

21

69

100

91

97

Photostability in HEK cells (low power, s)^b

25

57,500^c

996^c

2127^c

1466^c

Photostability in HEK cells (high power, s ± SEM)^b

710 ± 70

1080 ± 25

1150 ± 45

950 ± 45

610 ± 50

Relative brightness in cultured neurons^a

N.D

70

100

78

91

Photostability in neurons (s ± SEM)^b

N.D

635 ± 70

135 ± 5

185 ± 10

200 ± 5

Relative brightness in C. elegans^a

N.D

N.D

100

237

218

Relative brightness in zebrafish (hb)^a

N.D

N.D

100

82

117

Relative brightness in zebrafish (sc)^a

N.D

N.D

100

95

110

Photostability in zebrafish (sc, s ± SEM)^b

N.D

N.D

39 ± 2.2

28.6 ± 2.6

20.8 ± 1.6

Relative brightness 2photon^a

N.D

N.D

100

121

87

OSER assay^d

N.D

57 ± 2.7³¹

36.3¹²

49.8 ± 1.9³¹

68.9 ± 3.3^e

65.6 ± 4.6^e

61.2 ± 1.8^e

MFI:NE MFI ± SEM

N.D

2.6 ± 0.08

2.9 ± 0.1

2.7 ± 0.1

2.7 ± 0.09