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Figure 1

From: Methanol fixed feeder layers altered the pluripotency and metabolism of bovine pluripotent stem cells

Figure 1

Characterization of bovine ESCs grown on mitomycin C treated and methanol fixed feeder cells. (a) Schematic diagram shows the experimental strategy for analyzing bESCs grown on mitomycin C-treated mouse embryonic fibroblasts (MC-MEFs) and methanol-treated MEFs (MT-MEFs). (b) The morphology and AKP staining of MC-MEFs and the F7 grown on MC-MEFs (MC-F7). Bar = 100 μm. (c) The morphology and AKP staining of MT-MEFs and F7 grown on MT-MEFs (MT-F7). Bar = 100 μm. (d) Growth curves of MC-F7 and MT-F7 cells. (e) Real-time quantitative polymerase chain reaction (RT-PCR) analysis shows the expression of the key pluripotency regulatory factors OCT4, SOX2, and NANOG in MC-F7 and MT-F7 cells. (f) Western blotting analysis shows the protein expression of OCT4, SOX2 and CDX2 in MC-F7 and MT-F7 cells.

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