Figure 2 | Scientific Reports

Figure 2

From: Methanol fixed feeder layers altered the pluripotency and metabolism of bovine pluripotent stem cells

Figure 2

Immunofluorescence and RT-PCR analyses bESCs on methanol-fixed feeder layer. (a) Immunofluorescence staining images show the expression of SOX2, OCT4, NANOG, and CDX2 proteins in MC-F7 and MT-F7 cells. Bar = 100 μm. (b) RT-PCR analysis shows the expression levels of pluripotent markers such as KLF5, DNMT1, DNMT3A, DNMT3B, STELLA, TFCP2L1, and REX1 in the MC-F7 and MT-F7 cells.

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