Figure 3 | Scientific Reports

Figure 3

From: Methanol fixed feeder layers altered the pluripotency and metabolism of bovine pluripotent stem cells

Figure 3

The bovine ESCs cultured on methanol-fixed MEFs show long-term differentiation potential. (a) Schematic diagram shows the experimental strategy for differentiation of bESCs. (b,c) The embryoid bodies (EBs) genrerated from MC-F7 and MT-F7 cells. (d) The differentiated MT-F7 and MC-F7 cells show high expression levels of endodermal (AFP), mesodermal (SMA), and ectodermal (GFAP) marker proteins. Bar = 100 μm.

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