Figure 6
Characterization of central carbon metabolism in the F7 grown on MT-MEFs and MC-MEFs. (a) RT-PCR analysis showing the expression levels of the key catalytic enzymes, including rate-limiting enzymes in major metabolic pathways. PPP pentose phosphate pathway, PM pyruvate metabolism, FAS de novo fatty acid synthesis. (b) The relative expression of genes encoding catalytic enzymes related to pyruvate metabolism. (c) The relative expression of genes encoding catalytic enzymes related to glutaminolysis. (d) The relative expression of genes encoding catalytic enzymes related to acetyl CoA (AcCoA) biosynthesis (ACSS family). (e) The metabolic diagram shows the differential expression of genes mentioned above.
