Figure 7
Effects of the methanol-fixed feeder layer on the bEPSCs. (a) The schematic diagram shows experimental strategy for analyzing bEPSCs grown on MT-MEFs and MC-MEFs. (b) The morphology of MC-bEPSCs and MT-bEPSCs. Bar = 100 μm. (c) AKP staining of MC-bEPSCs and MT-bEPSCs. Bar = 100 μm. (d) Growth curve of MC-bEPSCs and MT-bEPSCs. (e) RT-PCR analysis shows the expression of SOX2, OCT4 and NANOG in the MC-bEPSCs and MT-bEPSCs. (f) Western blotting analysis shows the expression levels of OCT4, SOX2, and CDX2 in the MC-bEPSCs and MT-bEPSCs. (g) Immunofluorescence staining assay results show the expression levels of SOX2, OCT4, and CDX2 in the MC-bEPSCs and MT-bEPSCs. Bar = 100 μm.
