Figure 8
Characterization of central carbon metabolism in the bEPSCs grown on methanol-fixed MEFs. (a) RT-PCR analysis shows the expression levels of pluripotent regulatory genes, KLF2, KLF4, KLF5, DNMT1, DNMT3A, DNMT3B, STELLA and REX1, in the MC-bEPSCs and MT-bEPSCs. (b) RT-PCR analysis shows the expression levels of genes encoding key catalytic enzymes, HK1, PKM, LDHA, G6PD, IDH2 and CS, in major metabolic pathways. (c) RT-PCR analysis shows the expression levels of genes encoding the catalytic enzymes, MPC1, PC, PDHB, PCK2, ME1 and ME2, related to pyruvate metabolism. (d) RT-PCR analysis shows the expression levels of genes encoding the catalytic enzymes, ACLY, ACACA, FASN and SCD1, involved in de novo fatty acid biosynthesis. (e) The metabolic diagram shows differential expression of genes mentioned above.
