Figure 7

Overexpression of miR-628 and treatment of Enzalutamide or DTX decreases cell proliferation and induces apoptosis in LNCaP. (A) LNCaP cells transfected with miR-628 mimic or NC were treated with enzalutamide (ENZ) (5 µM, or 10 µM) or docetaxel (DTX) (5 nM or 10 nM). Cell viability was measured after 24 h and 48 h after transfection using MTT assay (*p < 0.05, **p < 0.01, ***p < 0.001). (B) The effect of miR-628 and co-treatment of ENZ or DTX on PCa cell apoptosis was assessed through cleaved-PARP and pro-Caspase-3 expression using western blotting as described in the materials and methods section. Membranes were cut before hybridization using the molecular weight marker as a reference and imaged using an Azure imaging system. All western blot full images are provided in supplementary information files. (C) Schematic model representing the anti-PCa role of miR-628. MiR-628 targets Jagged1 affecting Notch signaling and EMT.