Figure 3

(a) Cartoon representation of N-glycosylated 1HZH with the 16 disulfide bonds represented using the bond representation. Beads enclosed by a surface shows the locations of the four persistent Fc-Fab2 interactions involving LC2. (b) Heat map in the left panel shows the bound states of four long-lived Fc-Fab2 hydrogen bonds, in three independent 750 ns MD simulations. Bound states are displayed as filled regions. (c) Probability densities of \({R}_{25}\), \({\theta }_{125}\), and \({\Theta }_{1256}\) computed from 600 ns simulations of 1HZH-A2G0F in the absence of the hinge and Fab1 regions. Data shown for two independent replicates. Data from two independent replicates are shown using lines of similar colors.