Figure 5
NAS-IMI impairs specific lipids in the milk of water buffalos. (A) Cross-validation performed by the tenfold method to estimate the predictive ability of the generated models. Asterisk on model no. 5 indicates the highest and most consistent model (highest Q2, where Q2 estimates the model's predictive ability). (B) Variable importance in projection (VIP) scores of indicated metabolites listed in model no. 5. Blue and carmine red squares indicate low and high lipid levels. (C) Volcano plot representing down—(blue dots), upregulated (red dots), and unchanged lipids (grey dots) in NAS-IMI compared to H milk. Dark blue and carmine red dots indicate differentially abundant fatty acids. Significant lipids were considered with |log2 fold-change|> 1 and − log10 adjusted p value > 1.3.
