Figure 1 | Scientific Reports

Figure 1

From: A desirable transgenic strategy using GGTA1 endogenous promoter-mediated knock-in for xenotransplantation model

Figure 1

Ablation of GalT and targeted hCD55 and hCD39 gene at GGTA1 locus on produced GTKO/CD55/CD39 targeted cloned pig. (A) Schematic of targeting strategy to ablate GalT and knock-in of hCD55 and hCD39 gene. (B) Targeting PCR analysis in GTKO/CD55/CD39 transgenic pig (Donor; targeted colony, WT; wild-type, TG; transgenic pig). (C) Cell surface expression of GalT, hCD55, and hCD39 on variety cell source from wild type (blue line) and GTKO/CD55/CD39 transgenic pig (red line). (D) Comparison of hCD55 and hCD39 expression on GTKO/CD55/CD39 pig aorta endothelial cells (red line), and human aorta endothelial cells (blue line). Wild-type pig aorta endothelial cells (black line) was used as negative control. The original gels are presented in supplementary Fig. S3.

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