Table 2 Primer sequences used for quantitative real-time polymerase chain reactions (qRT-PCR) and their corresponding primer efficiency.

From: In vitro triple coculture with gut microbiota from spondyloarthritis patients is characterized by inter-individual differences in inflammatory responses

Gene

Forward primer sequence, 5′–3′

Reverse primer sequence, 3′–5′

Efficiency [%]

Reference

GAPDH

GGAGTCCACTGGCGTCTTCAC

GAGGCATTGCTGATGATCTTGAGG

92.33

61

ACTB

CTGGAACGGTGAAGGTGACA

AAGGGACTTCCTGTAACAATGCA

91.97

61

ZO-1

CGGTCCTCTGAGCCTGTAAG

GGATCTACATGCGACGACAA

98.87

62

TLR4

AATCTAGAGCACTTGGACCTTTCC

AGAAATCTGGACAGGGACTTGGG

103.29

63

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