Table 4 List of ten selected Anisakis pegreffii abundant miRNAs in larvae and in extracellular vesicles, together with putative orthologues miRNAs from other parasitic helminths with conserved seed region, and human miRNAs putative orthologues. The last two columns include Anisakis pegreffii miRNAs predictive targets in human genome according to miRDB and their ID according to NCBI database. Asterisks indicate a miRNAs enriched in exosomes, according to literature. (Underlined miRNAs are abundant both in L3 and in EVs list).
Anisakis pegreffii miRNAs | miRNAs in other helminths | Human miRNAs | Target—putative role | GeneID NCBI |
|---|---|---|---|---|
miR-100a-5p | asu-miR-100b-5p bma-miR-100a bma-miR-100b bma-miR-100c bma-miR-100d hpo-miR-100-5p* | hsa-miR-100-5p | TRIB2—interact and modulate the activity of signal transduction pathways in physiological and pathological processes. This Tribbles member induces apoptosis of cells mainly of the hematopoietic origin | 28951 |
miR-1-3p | asu-miR-1-3p str-miR-1-3p hco-miR-1-3p hpo-miR-1-3p fhe-miR-1-3p | hsa-miR-1-3p | MMD—monocyte to macrophage differentiation associated protein | 23531 |
miR-71-5p | asu-miR-71-5p bma-miR-71 str-miR-71-5p hpo-miR-71-5p hco-miR-71 | No match | NAA25—auxiliary subunit of the heteromeric N-terminal acetyltransferase B complex | 80018 |
miR-9-5p | asu-miR-9-5p bma-miR-9-5p str-miR-9-5p hco-miR-9 hpo-miR-9-5p | hsa-miR-9-5p | PRTG—protogenin, member of the immunoglobulin superfamily | 283659 |
lin-4-5p | asu-lin-4-5p bma-lin-4 str-lin-4-5p hco-lin-4 hpo-lin-4-5p cel-lin-4-5p | hsa-miR-125b | ARID3B—family of DNA-binding proteins with roles in embryonic patterning and cell lineage, cell cycle control, transcriptional regulation and chromatin structure modification STARD13—regulation of cytoskeletal reorganization, cell proliferation, cell motility, and acts as a tumor suppressor in hepatoma cells BMF—apoptotic activator FREM1—co-receptor of the interleukin 1 receptor family contributing to the control of inflammatory response activation | 10620 90627 90427 158326 |
miR-5358a-3p | asu-miR-5358a-3p | No match | TRIB2—interact and modulate the activity of signal transduction pathways in physiological and pathological processes. This Tribbles member induces apoptosis of hematopoietic cells | 28951 |
miR-5364-3p | asu-miR-5364-3p bma-miR-5364 | No match | STARD13—regulation of cytoskeletal reorganization, cell proliferation, cell motility, and acts as a tumor suppressor in hepatoma cells | 90627 |
novel-miR-19 | No match | hsa-mir-1322 hsa-mir-4502 | FAM83C—involved in regulating MAPK signaling in cancer cells | 128876 |
novel-miR-97 | No match | No match | LINGO1—leucine rich repeat and Ig domain | 84894 |
miR-5353-3p | asu-miR-5353-3p | No match | NECAP2—member of the adaptin-ear-binding coat-associated protein family. Studies of a similar protein in rat suggest a role in clathrin-mediated endocytosis | 55707 |
