Figure 2
High-throughput widefield P-SHG imaging of tissue microarray. (a) A H&E-stained breast tissue microarray containing normal and three distinct tumor subtypes. Enumeration indicates the number assigned to each breast tissue core. The positive sign indicates overexpression of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2), denoted by ER/PR/HER2. (b) Image of circularly-polarized SHG intensity of the entire microarray slide shows distinctive low signal associated with tumor cores, except cores 21 and 22 that possess a large amount of collagenous stroma. Scale bar: \(2.5 \; \text {mm}\). (c) A single widefield SHG image consisting of \(2048 {\times } 2048\) pixels and an area of approximately \(670 \; \upmu \text {m} {\times } 670\; \upmu \text {m}\). Scale bar: \(200 \; \upmu \text {m}\). (d) A magnified region of the tissue, highlighted by red rectangle in c shows high resolution of the imaging technique. Scale bar: \(25 \; \upmu \text {m}\).
