Table 6 Results of purification of NanB sialidase by anion exchange chromatography and affinity chromatography.

From: Screening and purification of NanB sialidase from Pasteurella multocida with activity in hydrolyzing sialic acid Neu5Acα(2–6)Gal and Neu5Acα(2–3)Gal

NanB sialidase isolation method

Supernatant volume (ml)

Total protein (mg)

Total sialidase activity (U)

Specific activity (U/mg)

Sialidase activity (U/ml)

Purification fold

Crude protein

100

64.80

16.80

0.259

0.168

1

Anion exchange chromatography

100

8.60

15.40

1.791

0.154

6.9

Affinity chromatography

15

0.372

3.87

10.40

0.258

40.13

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