Figure 1 | Scientific Reports

Figure 1

From: HIF-1α-mediated augmentation of miRNA-18b-5p facilitates proliferation and metastasis in osteosarcoma through attenuation PHF2

Figure 1

miR-18b-5p is upregulated in OS and associated with poor clinical outcome. (a) Heatmap of microarray data obtained from GEO datasets (GSE28423) showed 60 most upregulated and downregulated miRNAs in OS cells and normal osteocytes. The image was created by RStudio (pheatmap). (b) The volcano plot showed differential expressed miRNAs (log2 fold change > 2, p < 0.001). The image was generated by RStudio (ggplot2). (c) RT-qPCR assay showed the miR-18b-5p expression in 7 OS cell lines compared with normal bone cells (hFOB1.19). (d) RT-qPCR assay showed the miR-18b-5p expression in 60 OS tissues and paired adjacent normal tissues (NB). (e) Kaplan–Meier curves analysis displayed the percentage survival of 60 OS patients in high and low miR-18b-5p groups. The 50th percentile of miR-18b-5p expression was a cutoff point divided into “high” and “low”.

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