Figure 2 | Scientific Reports

Figure 2

From: HIF-1α-mediated augmentation of miRNA-18b-5p facilitates proliferation and metastasis in osteosarcoma through attenuation PHF2

Figure 2

Upregulation of miR-18b-5p accelerates development of OS. (a) RT-qPCR was performed to detect miR-18b-5p expression in MG-63/143b cells transfected with miR-18b-5p mimic or miR-NC. (b) The cell viability of MG-63/143b cells was evaluated by the MTT method. (c,d) The capacity of invasion and migration of MG-63/143b cells was valuated by the transwell assay. Scale bar = 100 μm. (e) Xenograft tumor of nude mice subcutaneously injected with MG-63 with stably miR-18b-5p overexpression. (f,g) The weight and volume of xenograft tumor of nude mice. (h–j) The nude mice was injected with MG-63 with stably miR-18b-5p overexpression from tail vein. The number of lung metastasis of nude mice counted via H&E staining (h). The images of H&E staining of lung metastasis (i). The survival of nude mice was analyzed (j).

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