Figure 3 | Scientific Reports

Figure 3

From: HIF-1α-mediated augmentation of miRNA-18b-5p facilitates proliferation and metastasis in osteosarcoma through attenuation PHF2

Figure 3

miR-18b-5p modulates the expression of PHF2 through post-transcriptional regulation. (a) Venn diagram showed the predicted targets of miR-18b-5p from databases (miRanda, miRmap, microT and PicTar). The image was created using Online tools of Bioinformatics Evolutionary Genomics (http://bioinformatics.psb.ugent.be/webtools/Venn/). (b) Schematic diagram showed the predicted interaction between 3′-UTR-PHF2- mRNA and miR-18b-5p. The mutated sequences of the 3′-UTR-PHF2- mRNA was displayed. (c,d) RT-qPCR assay showed the expression of PHF2 mRNA in MG-63/143b cells with miR-18b-5p overexpression or inhibition. (e,f) Western blotting assay showed the protein level of PHF2 in miR-18b-5p upregulated or downregulated MG-63 and 143b cells. (g) Biotin-based pulldown assay detected the enrichment of PHF2 on biotinylated miR-NC and miR-18b-5p probe with the mRNA of GAPDH acted as control. (h,i) The luciferase reporter assay accessed luciferase activity of miR-18b-5p upregulated or downregulated MG-63 and 143b cells transfected with luciferase reporter plasmid carrying WT-3′-UTR-PHF2 or MUT-3′-UTR-PHF2.

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