Figure 4

Triacylglycerol synthesis pathway for female seasonal differential expression. Emu triacylglycerol synthesis is represented by the Gallus gallus pathway, which is fuelled by the fatty acid synthesis pathway to provide fatty acyl-CoA metabolites. Metabolites (grey oval nodes) are catalysed (black solid arrows) by gene products (rectangle nodes). Dashed arrows represent a process that involves additional steps not displayed in the diagram. Gene products are split into thirds and correspond to the month-wise comparisons from the female seasonal differential expression analysis. Each third is coloured by their respective gene’s Benjamini–Hochberg corrected p-value in a gradient from more significant (red) to less significant (blue). White represents genes that have non-substantial p-values calculated by DESeq2²¹, while grey represents no annotation available for emu or chicken. Three seasonally DE genes (DGAT2, LPL, and MOGAT2) have bar plots of expression levels in median log₂(TPM) by month featuring median absolute deviation error bars. Numerical Benjamini–Hochberg corrected p-values are shown above the horizontal brackets of the bars that indicate the month-wise comparison, where an asterisk to the right represents statistical significance (p < 0.01).