Table 1 Comparison of the targeting efficiency using hyb-gRNA (mRNA/hyb-gRNA) and Cas9 mRNA with Cas9 protein and sgRNA (Nuc/sgRNA).

From: CRISPR/Cas9 editing of the MYO7A gene in rhesus macaque embryos to generate a primate model of Usher syndrome type 1B

Injection material

# Total oocytes

# Fertilized oocytes (%a)

# Arrested embryos (%b)

Blastocysts (%c)

# Embryos analyzedd

# Embryos edited (%e)

# Fully edited embryosf (%g)

mRNA/hyb-gRNA

125

53 (42.4%)

33 (62.26%)

11 (20.75%)

36

14 (38.88%)

4 (28.5%)

Nuc/sgRNA

409

234 (57.21%)

200 (85.47%)*

26 (11.11%)

211*

161 (76.3%)*

105 (65.21%)*

  1. *p < 0.01, Nuc/sgRNA versus Cas9 mRNA/hyb-gRNA.
  2. a% = number of cleavage stage embryos/total number of oocytes that underwent IVF X 100.
  3. b% = number of arrested embryos/number of fertilized oocytes X100.
  4. c% = number of blastocysts/number of fertilized oocytes X 100.
  5. dIncludes both arrested embryos and TE cell biopsy samples from blastocysts.
  6. e% = number of any edited embryo biopsies and arrested embryos/total number analyzed X 100.
  7. fIncludes those arrested embryos and TE biopsies that possess 100% mutated sequence reads.
  8. g% = number of 100% mutated sample/embryo edited X 100.
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