Figure 3
From: Immuno-reactive cancer organoid model to assess effects of the microbiome on cancer immunotherapy
Characterization of iTOs. (a) H&E stained iTO shows immune cell co-localizing to cancer cells (purple arrow indicates splenocytes and red indicates cancer cells. 20 × magnification; inset 4 × magnification). (b) Flow cytometry analysis of broad FSC-A vs. SSC-A, CD45+CD3+, and CD45+CD3+CD8+cells isolated from the iTOs., as indicated (c) Viability of CD45+CD3+CD8+ cell fraction where the Y-axis is cell count and the X-axis is fluorescent intensity (n = 3 organoids).
