Figure 4
From: Immuno-reactive cancer organoid model to assess effects of the microbiome on cancer immunotherapy
The effects of ICB on immune cell cytotoxicity in iTOs. ITOs were treated with anti-CTLA-4 and anti-PD-1, ICB combination, as indicated, and analyzed for CD8, granzyme B, and cleaved caspase 3 expression with immunostaining. (a, b) DAPI normalized CD8-positive (a) and granzyme B-expressing (b) cell numbers are significantly increased by ICB administration in iTOs. (p = 0.0158 and 0.0024 for CD8 and granzyme B, respectively, n = 4). Representative IF images from each group are shown below. (c) Cleaved caspase-3 IF signal in 4T1 cell only organoids, splenocytes only organoids and iTOs, treated with ICB and untreated, as indicated (significance in splenocytes only organoids p = 0.0021) (n = 4 ROI).
