Figure 4
From: Coplanar embedding of multiple 3D cell models in hydrogel towards high-throughput micro-histology
HistoBrick preparation and use. (a) Place the silicone mould in a glass petri dish with the pillars facing downwards and fill the mould with the agarose-HistoGel mixture from the side openings, (b) The filled mould should then be cooled at 4–5 °C for 2 h, (c) Unmold the HistoBrick by pressing the sides, (d) Unmolded HistoBrick, (e) Load fixed micro-tissues, (f) Add HistoGel in the wells and cool at 4–5 °C, (g) Cut the block using the slicing tool, (h) After dividing the HistoBrick into three segments, the two side segments can be discarded and the central segment containing the wells should be reserved for histological processing, (i) Place the central segment of HistoBrick in a histology cassette.
