Figure 5
From: Coplanar embedding of multiple 3D cell models in hydrogel towards high-throughput micro-histology
Images of HepG2 micro-tissues in a HistoBrick undergoing different processing steps. (a) A HepG2 micro-tissue (pointed by the arrow) after being embedded in the HistoBrick and topped with HistoGel. Note no air bubbles are trapped inside the micro-well, (b) H&E staining of a section of a single micro-tissue, (c) Microscopy image of a slice without dye. The well contours are difficult to determine. (d) Microscopy image of a slice using a blue coloured stain. Note the well contours are clearly visible. (e) Measuring the diameter and pitch of the wells in the HistoBrick before paraffin embedding, (f) Measuring the diameter and pitch of the wells in the HistoBrick after sectioning. The parameter D is the diameter whereas P1 and P2 are used to compare the pitch on the two axes of the micro-well array.
