Figure 6
From: Coplanar embedding of multiple 3D cell models in hydrogel towards high-throughput micro-histology
Coplanarity of embedded HepG2 micro-tissues. (a) Bright-field images of three H&E-stained slides from the same processed block. Light disks correspond to wells and dark dots correspond to micro-tissues, as in the example circled with dotted line, (b) Schematic of the position of the spheroids in the HistoBrick. Best fitting plane (yellow line) is an imaginary plane to compensate the tilting angle (in red, the angle formed by the best fitting plane with the plane of the microtome blade) which is the result of manual positioning of the block on the microtome, (c) Distance of micro-tissues’ centre from the first histology section for seven different HistoBrick samples [5 Histobricks were cut without alignment tool (blue boxes) and two HistoBricks were cut with alignment tool (green boxes)]. (d) Absolute distance of the micro-tissues’ centre from the best fitting plane for the same seven HistoBrick samples.
