Figure 1
Liproxstatin-1-primed hUC-MSCs alleviated airway inflammation mitigated pulmonary fibrosis in vivo. (a) Shows the result of total cell counts and (b) shows differential counts of macrophages, neutrophils, eosinophils, and lymphocytes in BAL fluid. (c) Shows microscopic images of Diff-Quick-stained slides of cytocentrifuged BAL fluid (× 40). Samples from IL-13 TG groups were diluted with doubled amount of PBS compared with the samples from the wild type groups. (d–e) shows histological comparison between the groups at a power of 20. H&E (d) and PAS (e) stains were used. (f) Shows Masson’s Trichrome-stained slides of each group at low power (× 20). (g–h) shows semi-quantitative grading results of H&E staining (g) and PAS staining (h). Sircol collagen assay was performed to quantify the soluble collagen contents in the homogenates of the lungs (i). (j–n) represents relative gene expressions of Muc5ac (j), Fgf1 (k), Fn1 (l), Mmp-9 (m), and Mmp-12 (n) compared to the housekeeping, Gapdh. Each value in these panels is from a different individual and the mean ± SEM are illustrated. All results are representative of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns not significant. (by Kruskal–Wallis test using GraphPad Prism 7, https://www.graphpad.com).
