Figure 2

Liproxstatin-1-primed hUC-MSCs altered macrophage populations in the lung. (a) Flow cytometry data showing two distinct macrophage populations that differ by levels of CD11b and F4/80 expression. Numbers represent percentages among total CD45⁺ leukocytes, excluding SiglecF⁺CD11c⁻ eosinophils. Based on the flow cytometry representations shown in (a), percentages of CD11b^highF4/80^int macrophages (b) and CD11b^intF4/80^high macrophages (c) are each portrayed in graphs. (d) Represents a histogram of Ly6C and CX₃CR1 expression levels of CD11b^intF4/80^high macrophages and CD11b^highF4/80^int macrophages. (e) Represents the MFI of Ly6C of CD11b^highF4/80^int macrophages and (f) shows the MFI of CX₃CR1 of CD11b^intF4/80^high macrophages. (g) Shows the percentages of Ly6⁺ macrophages among CD11b^highF4/80^int macrophages. Flow cytometry data were analyzed by the FlowJo Software v10.6, https://www.flowjo.com. Each value in these panels is from a different individual and the mean ± SEM are illustrated. All results are representative of at least three independent experiments. *p < 0.05, ns not significant. (by Kruskal–Wallis test using GraphPad Prism 7, https://www.graphpad.com).