Figure 4

Ly6C⁺M2 macrophages are key mediators of asthmatic phenotypes. (a) Flow cytometric data on M1 and M2 macrophages of CD11c⁺CD11b⁺ macrophages. The percentages of CD86⁺CD206⁻ M1 (b), CD86⁻CD206⁺ M2 macrophages (c), and CD86⁺CD206⁺ macrophages (d) are represented in scatter plots. (e) the MFI of IL-5 in CD86⁺CD206⁻ M1 and CD86⁻CD206⁺ M2 macrophages in IL-13 TG mice. (f) Shows Ly6C expression levels in M1 and M2 macrophages of WT and IL-13 TG mice. (g, h) shows the ratio of Ly6C⁺M2 and LY6C⁻M2 macrophage populations among the CD11c⁺CD11b⁺ macrophages. Flow cytometry data were analyzed by the FlowJo Software v10.6, https://www.flowjo.com. Each value in these panels is from a different individual and the mean ± SEM are illustrated. All results are representative of at least three independent experiments. *p < 0.05, **p < 0.01, ns not significant. (by Kruskal–Wallis test using GraphPad Prism 7, https://www.graphpad.com).