Figure 1
From: Imaging peripheral nerve micro-anatomy with MUSE, 2D and 3D approaches
2D-MUSE image of a rat sciatic nerve. Fixed nerve tissue was sectioned on a compresstome and laid immediately on a quartz slide for imaging. Individual myelinated fibers are visible (b and c) with contrast originating from tissue autofluorescence under UV illumination. (d) Manual segmentation on a subset of myelinated fibers in (b) allows quantification of axon diameters and corresponding g-ratios, displayed as histograms in (e). Scale bars are: (a) 100 µm, (b and c) 50 µm. Close-up views in (b) and (c) were contrast enhanced to improve visibility of myelin sheaths.
