Figure 2
From: Imaging peripheral nerve micro-anatomy with MUSE, 2D and 3D approaches
2D-MUSE image of a rat sciatic nerve with staining. Sample was sectioned by hand with a razor blade, immersed in a rhodamine and Hoechst staining solution for 30 s, rinsed in DI water and imaged. (a) Transverse cross-section illustrates several myelinated fibers grouped as a single fascicle and surrounded by a thin perineural sheath. (b and c) Close up views from (a) show multiple myelinated fibers as yellow rings. (d) Longitudinal cross section allows visualizing fibers along the length of the nerve. (e) Zoomed in region from (d) illustrates microvasculature revealed by Hoechst staining and evident from tubular structure (arrows). (f) A gap in the myelinated fibers is visible in this close-up view, suggestive of Schimdt-Lanterman clefts or node of Ranvier (asterisk). Scale bars are: 100 µm (a, d) and 20 µm (b, c, e, f).
