Figure 4
From: Imaging peripheral nerve micro-anatomy with MUSE, 2D and 3D approaches
Imaging a cadaver tibial nerve sample with block-face 3D-MUSE imaging. The nerve was post-fixed with osmium tetroxide and counterstained with rhodamine B. Images were acquired with a 10 × 0.45 NA objective and the sample was sectioned at 5 µm thickness. Further details of the system are provided in “Methods”. (a) Representative block face image stitched from twelve individual tiles. Structures of interest include fascicles, venuole (asterisk), and adipose cells (plus). (b and c) Close up views indicate individual fascicles and myelinated fibers respectively. (d) Volume rendering of a region within a fascicle, outlined by a brown box in (a). (e) Manual segmentation of intraneural vasculature from the same volume as in (d). Box size: 800 × 600 µm2. Scale bars are (a) 1 mm (d and e) 250 µm.
