Figure 3
From: Thermal gradient ring reveals thermosensory changes in diabetic peripheral neuropathy in mice
Expression of TRPV1 and TRPA1 in the dorsal root ganglia of mice 2 and 5 weeks after STZ administration. (a,b) mRNA expression levels of TRPV1 (a) and TRPA1 (b) in dorsal root ganglia (DRG) at 2 (left) and 5 (right) weeks after STZ administration. We used 36B4 as a housekeeping gene to normalize the expression. WT (non-DM), n = 7–10. WT (DM), n = 8–10. (c) Comparison of protein levels of TRPV1 in DRG by western blot analysis at 2 (left) and 5 (right) weeks after STZ administration. We used β-actin as an internal loading control. The predicted molecular weights of TRPV1 and β-actin are 95 and 42 kDa, respectively. WT (non-DM), n = 5–6. WT (DM), n = 6. The data are presented as the means ± SEM. A two-tailed t test was used for comparisons. *p < 0.05 and **p < 0.01. ns = not significant. Open circles indicate data.
