Figure 5

Thermal behavior assay with the Thermal Gradient Ring. (a) Image showing the Thermal Gradient Ring with a mouse (left) and an image of the floor of the Thermal Gradient Ring with 24 zones from 10 to 55 °C (right). (b) Comparison of “spent time” of 4 groups (WT (DM) mice 5 weeks after STZ administration (n = 10), and WT (non-DM) (n = 10), TRPV1^−/− (non-DM) (n = 10), TRPA1^−/− (non-DM) (n = 9) mice at the same age) during 40–60 min in the 60 min trial. Expanded data at 40–55 °C zones are also shown (b, right). (c) Mean values of preferred temperatures calculated from “spent time” and zone temperatures of (b), in the first, mid, and last 20 min. (d) Comparison of “spent time” of 3 groups (WT (DM) mice 2 weeks after STZ administration (n = 8), and WT (non-DM) (n = 10), TRPV1^−/− (non-DM) (n = 5) mice at the same age) during 40–60 min in the 60 min trial. Expanded data at 40–55 °C zones are also shown (b, right). (e) Mean values of preferred temperatures calculated from “spent time” and zone temperatures of (d), in the first, mid and last 20 min. The data are presented as the means ± SEM. A one-way ANOVA followed by a Bonferroni post hoc test was used for comparisons. *p < 0.05 and **p < 0.01. ns = not significant.