Table 1 Identification of PTH derivatives based on their retention times, m/z values, and CD absorption at 270 nm.
Amino acid | m/z values of PTH derivative | Retention time of PTH derivative (min) | CD absorption of PTH derivative | |
|---|---|---|---|---|
l-amino acid | d-amino acid | |||
Ala | 207 | 14.60 | + | − |
Arg | 292 | 3.52 | + | − |
Asn | 250 | 4.22 | + | − |
Asp | 265 | 16.67 | + | − |
Cys | 223 | 4.76 | + | − |
Gln | 264 | 6.98 | + | − |
Glu | 279 | 23.42 | + | − |
Gly | 193 | 6.18 | ||
His | 273 | 1.81 | + | − |
Ile | 249 | 42.60 | + | − |
Leu | 249 | 43.78 | + | − |
tert-Leu | 249 | 43.11 | + | − |
Lys | 399 | 46.08 | + | − |
Met | 267 | 29.98 | + | − |
Phe | 283 | 44.98 | + | − |
Pro | 233 | 26.33 | − | + |
Ser | 223 | 4.82 | + | − |
Thr | 237 | 12.49 | + | − |
Trp | 322 | 44.30 | + | − |
Tyr | 299 | 21.82 | + | − |
Val | 235 | 29.01 | + | − |
