Figure 1

Cdh1 deficiency leads to inefficient fetal erythropoiesis and anemia at birth. (A) Red cell parameters from peripheral blood of control (Cdh1 WT) and Cdh1-deficient (Cdh1 KO) pups collected 12 to 18 h after birth. The scatter dot plots show mean ± s.e.m of the corresponding data (n = 15 for WT and 12 for KO). (B) Erythroid maturation was assessed based on expression of Ter119 and CD71 cell surface markers in fetal livers of the indicated embryonic stage and genotype. The ratios of pro-erythroblasts (Ter119^- CD71⁺), immature erythroblasts (Ter119⁺ CD71⁺), and mature erythroblasts and reticulocytes (Ter119⁺ CD71^low/-) were determined in 4–7 samples for each stage and genotype (bar graphs, right). Representative flow cytometry plots are included on the left. (C) Quantification of the percentage of enucleated erythroid cells (Ter119⁺ Draq5^-) in fetal livers from the indicated embryonic stage and genotype. The top panels show representative flow cytometry profiles. n = 10 for E13.5; n = 6 for E17.5; *P < 0.05, **P < 0.01, ***P < 0.001.