Figure 2

Signs of anemia and impaired terminal erythroid differentiation in adult mice lacking Cdh1 expression in hematopoietic cells. (A) Red cell parameters from peripheral blood of 8–12 week old mice of the indicated genotype. The scatter dot plots show mean ± s.e.m of the corresponding data. (n = 51 for Vav-Cdh1 WT and 46 for Vav-Cdh1 KO). (B) Relative weight of the spleen in control (Vav-Cdh1 WT) and mutant (Vav-Cdh1 KO) adult mice (n = 10). A representative picture is included (left). (C, D) Terminal erythroid maturation was assessed based on CD71 expression in Ter119⁺ gated cells in the bone marrow (C) and spleen (D) from adult mice of the indicated genotype. The relative abundance of populations showing high, low, or no CD71 expression, representing increasingly higher maturation stages, were quantified (bottom graphs). Representative flow cytometry plots are included (top panels). (n = 6). *P < 0.05, **P < 0.01, *** P < 0.001.