Figure 6
Accumulation of DNA damage in Cdh1-deficient maturing erythroblasts. Fetal liver cells from control (Cdh1-WT) and Cdh1-null (Cdh1-KO) E17.5 embryos were sorted based on Ter119 and CD71 expression and then stained for EdU, ɣH2AX and DNA content (n = 4 for each genotype). (A) Quantitation of cells with strong ɣH2AX staining in the indicated sorted erythroblast populations. Left panels show representative flow cytometry plots and include the gate used for quantitation (black box). (B) Within each of the sorted erythroblast populations, the percentage of cells positive for ɣH2AX and EdU (right column graph) or positive for ɣH2AX but negative for EdU (left column graph) was measured. The representative flow cytometry plots (left panels) show the corresponding gating. **P < 0.01, ***P < 0.001, ns (not significant) P > 0.05.
